J Anesth Perioper Med. 2019;6(X):XXX-XXX. https://doi.org/10.24015/ebcmed.JAPM.2019.0007

Altered Expression of 5-HT Receptor Subtypes in the Spinal Cord After Cardiac Ischemia-Reperfusion Injury

Zhi-Xiao Li1, Zhi-Gang He1, Shun-Yuan Li2, and Hong-Bing Xiang1

From the 1Department of Anesthesiology and Pain Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; 2Department of Anesthesiology, The First Affiliated Quanzhou Hospital of Fujian Medical University, Quanzhou, China.

Correspondence to Dr. Hong-Bing Xiang at xhbtj2004@163. com or Dr. Shun-Yuan Li at cylfj@126.com.

EBCMED ID: ebcmed.JAPM.2019.0007 DOI: 10.24015/ebcmed.JAPM.2019.0007


Abstract

Background
Spinal cord plays an important role in the development and progression of cardiac ischemia-reperfusion injury (CIRI).Numerous studies have been performed to identify the specific role of cardiac 5-hydroxytryptamine (5-HT) in cardiomyocyte damage, whereas reports about the potential role of spinal 5-HT during cardiac ischemia are rare. We investigated the involvement of 5-HT receptor subtypes in the spinal cord after CIRI.

Methods
Male adult SD rats (250 ~ 300 g) were randomly divided into two groups: Control group (Sham, n = 10) and Model group (30 min ischemia followed by 2 h reperfusion, n = 10). The animals of Control group had the same operation (thoracotomy) as the Model group but without left anterior descending coronary artery ligation. By using real-time quantitative polymerase chain reaction (RT-qPCR), we drew the expression profiles of 5-HT receptor subtypes in the upper thoracic segment of the spinal cord after CIRI.

Results
We found that there was a significant decrease of 5-HT1A, 5-HT1B, 5-HT1D, 5-HT3C, 5-HT5B, and 5-HT6 expression after CIRI, indicating that the specific role of 5-HT receptor subtypes in the spinal cord after CIRI.

Conclusion
Our results will be beneficial for understanding the serotonergic drug targets to decrease CIRI in future preclinical and clinical studies. (Funded by the the National Natural Science Foundation of China, National Natural Science Foundation of Hubei Province, and Medical innovation project in Fujian Province, all in China.)

Article Type
Original Article

Declaration of Interests
The authors have no other potential conflicts of interest for this work.

Acknowledgements
This work was supported by grants from the National Natural Science Foundation of China (81670240, 81873467), National Natural Science Foundation of Hubei Province (2016CFB625), and Medical Innovation Project in Fujian Province (2017-CX-48), all in China.

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